Silica SEC columns

TSKgel SW-type columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Three different pore sizes are available.

125 Å pore size for analysis of small proteins and peptides
250 Å pore size for most protein samples
450 Å pore size for very large proteins and nucleic acids

What sets TSKgel SW-type columns apart from the competition? TSKgel SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution.

Particle sizes for the three TSKgel SW-type column lines are:

TSKgel SuperSW: 4 micron
TSKgel SWXL: 5-8 micron
TSKgel SW: 10-13 micron

See each of the TSKgel SW-type product lines for representative calibration curves.

Highlights

TSKgel SW-type packings are comprised of rigid spherical silica gel particles chemically derivatized with diol-containing ligands.
Low adsorption and well-defined pore size distribution, which are requirements for high performance GFC of proteins.
Three pore sizes are available in TSKgel SW or SWXL product lines.
TSKgel SuperSW columns feature a 4 µm particle size packing for the best resolution and highest sensitivity.
Semi-preparative (21.5 mm ID) and preparative (55 mm, 108 mm ID) stainless steel columns are available for precise scale-up to commercial production.
PEEK and glass column hardware are available in the TSKgel SWXL product line

TSKgel SuperSW

TSKgel SWXL

TSKgel SW

TSKgel SuperSW columns contain spherical 4 µm particles (125 Å and 250 Å only) packed in 4.6 mm ID x 30 cm L columns.

For R&D.

High sensitivity when limited in sample mass.

2.6-fold reduction in solvent consumption.

Use 5 or 8 µm particle size TSKgel SWXL columns to improve sample resolution or to reduce analysis time (over SW columns).

For QC of mAb.
Select 7.8 mm ID x 15 cm L columns for high speed analysis.
Use PEEK columns to reduce sample adsorption to stainless steel or glass.

Analytical TSKgel SW column line features 10 or 13 µm particle sizes for improved sample capacity.

For protein isolation.

Select from stainless steel or glass column hardware.

Use preparative columns up to 21.5 mm ID for larger scale purifcations.

General Principles of
Chromatography - Mode Poster

Characterizing Biotherapeutics with HPLC - smart solutions for large molecules - Application note

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