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Tosoh Bioscience GmbH: The Chemistry of Innovation
Size Exclusion: Gel Filtration Chromatography
TSK-GEL Columns
Proteins (biopolymers)
TSKgel SW
TSKgel SWXL
TSKgel SuperSW
TSKgel UltraSW
Organic-soluble Polymers
Water-soluble Polymers
Polar Organic-Soluble Polymers

TSKgel SW-type columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Four different pore sizes are available.

  • 125 Å pore size for analysis of small proteins and peptides
  • 250 Å pore size for most protein samples
  • 300 Å pore size for mAb aggregates 
  • 450 Å pore size for very large proteins and nucleic acids

What sets TSKgel SW-type columns apart from the competition? TSKgel SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution.

Particle sizes for the TSKgel SW-type column lines are:

  • TSKgel UltraSW: 3 micron
  • TSKgel SuperSW: 4 micron
  • TSKgel SWXL: 5-8 micron
  • TSKgel SW: 10-13 micron

See each of the TSKgel SW-type product lines for representative calibration curves.

 

 Highlights
  • TSKgel SW-type packings are comprised of rigid spherical silica gel particles chemically derivatized with diol-containing ligands. 
  • Low adsorption and well-defined pore size distribution, which are requirements for high performance GFC of proteins. 
  • Three pore sizes are available in TSKgel SW or SWXL product lines. 
  • TSKgel SuperSW columns feature a 4 µm particle size packing for higher resolution and sensitivity.
  • TSKgel SuperSW mAb columns are optimized for antibody separation.
  • TSKgel UltraSW Aggregate are optimized for analysis of higher aggregates
  • Semi-preparative (21.5 mm ID) stainless steel columns are available for precise scale-up to commercial production. 
  • PEEK and glass column hardware are available in the TSKgel SWXL product line
TSKgel UltraSW TSKgel SuperSW TSKgel SWXL TSKgel SW
TSKgel UltraSW columns contain spherical 3 µm particles (300 Å pore size). TSKgel UltraSW Aggregate has a slightly larger pore size than the TSKgel SuperSW mAb columns and provides a wider separation window for higher mAb aggregates.

 

TSKgel SuperSW columns contain spherical 4 µm particles (125 Å and 250 Å only) packed in 4.6 mm ID x 30 cm L columns.

• High sensitivity when limited in sample mass. 
TSKgel Super SW mAb HTP  designed for fast UHPLC mAb analysis, features 250 Å pore size in 4.6 mm ID x 15 cm.
TSKgel Super SW mAb HR designed for high resolution mAb analysis, feature 250 Å pore size in 7.8 mm ID x 30 cm.

Use 5 or 8 µm particle size TSKgel SWXL columns to improve sample resolution or to reduce analysis time (over SW columns).

• For QC of mAb.
• Select 7.8 mm ID x 15 cm L columns for high speed analysis.
• Use PEEK columns to reduce sample adsorption to stainless steel or glass.

Analytical TSKgel SW column line features 10 or 13 µm particle sizes for improved sample capacity.

For protein isolation.
Select from stainless steel or glass column hardware.
Use preparative columns up to 21.5 mm ID for larger scale purifcations.



General Principles of
Chromatography - Mode Poster


Characterizing Biotherapeutics with HPLC - smart solutions for large molecules - Application note

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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com