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Tosoh Bioscience GmbH: The Chemistry of Innovation
Size Exclusion: Gel Permeation Chromatography
TSK-GEL Columns
Proteins (biopolymers)
Organic-soluble Polymers
Water-soluble Polymers
Polar Organic-Soluble Polymers
TSK-GEL Alpha
TSK-GEL SuperAW

TSKgel Alpha and SuperAW columns were developed for the GPC analysis of polymers of intermediate polarity. As in the PW- and PWxl-type resins, the particles in TSKgel Alpha and SuperAW columns have a hydroxylated methacrylate polymer backbone, but they are crosslinked to a higher degree to minimize swelling in polar organic solvents.

TSKgel Alpha and SuperAW particles are available in a wide variety of pore sizes.

Use semi-micro TSKgel SuperAW columns

  • for high-throughput applications
  • to reduce solvent consumption
  • to reduce solvent disposal cost.

Use TSKgel Alpha columns

  • when analysis time is not critical
  • when sample mass is not limited
  • to collect fractions
  • to obtain maximum number of plates (at the expense of analyis time).

 

 Highlights

TSKgel Alpha and SuperAW columns:

  • Particle sizes
    • TSKgel SuperAW, 4-9 μm particles
    • TSKgel Alpha, 7-13 μm particles
  • No or minimal swelling in a wide range of polar organic solvents: methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP.
  • For high thoughput applications, TSKgel SuperAW columns provide equivalent resolution in 1/2 the time compared to TSKgel Alpha columns.

  • Accurate MW determination and normal retention of polystrene polymers in DMF solvent.

  • System peaks from salts in the eluent elute away from the oligomer of interest.

Applications: polymers that are soluble in methanol, acetonitrile, DMSO, isopropanol, or THF; can also be used for water-soluble polymers.

TSKgel SuperAW

TSKgel Alpha

TSKgel SuperAW column line consists of five columns and a mixed-bed column.

High efficiency TSKgel SuperAW columns are available in 6.0 mm ID x 15 cm L column format for high throughput applications.

TSKgel Alpha column line consist of five columns and a mixed-bed column.

TSKgel Alpha columns are available in conventional 7.8 mm ID x 30 cm L column formats.


General Principles of
Chromatography - Mode Poster


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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com