TSKgel H-type columns p2

TSKgel GPC columns are available in conventional 7.8 mm ID x 30 cm L Hxl and Hhr columns and in high efficiency, high throughput 6 mm ID x 15 cm L SuperH and SuperHZ columns. How the product lines compare to one another is shown below.

Comparison of TSKgel H-type resins
Application focus	High-throughput polymer analysis with ultra low polymer adsorption, limited solvent compatibility range.	Conventional polymer analysis with ultra low polymer adsorption, limited solvent compatibility range	High-throughput polymer analysis with expanded solvent compatibility range.	Conventional polymer analysis with expanded solvent compatibility range.
Particle size	3, 5, and 10 micron, depending on pore size	5 and 9 micron, depending on pore size	3 and 5 micron, depending on pore size	5 micron
Theoretical plates ¹	16,000/15 cm column	16,000/30 cm column	16,000/15 cm column	16,000/30 cm column
Maximum temperature	G1000--G3000, 60°C G4000--Mixed, 80°C	G1000--G3000, 60°C G4000--Mixed, 80°C	140°C	140°C
Standard shipping solvent	THF	THF²	THF	THF²
THF can be switched to	see table 1 (below) and note #3 for dichloromethane and dichloroethane		see table 1 (below)
Other shipping solvents available?	yes⁴		no
Number of solvent substitutions	One time only		Several⁵
Solvent exchange instructions	Linear gradient with a 2%/min rate of change with a flow rate of <0.25 mL/min.	Linear gradient with a 2%/min rate of change with a flow rate of <0.5 mL/min.	Linear gradient with a 2%/min rate of change according to flow rates in table 2 (below).

1) Theoretical plates listed are based on smallest particle size listed.
2) High-temperature columns (HT) are shipped with ODCB (o-dichlorobenzene) as standard shipping solvent.
3) Dichloromethane and dichloroethane are not available for G1000 pore size columns.
4) See Table 1 (below) for available shipping solvents
5) After switching to a very polar solvent such as acetone, switching back to a nonpolar solvent is not recommended.

Table 1: Solvent compatibility for TSKgel H-type columns

The column shipping solvent limits the types of solvents that can be used with each column type.

TSKgel SuperHZ and Hxl¹

Tetrahydrofuran³

benzene, chloroform, toluene, xylene, dichloromethane, dichloroethane

Acetone**

carbon tetrachloride⁵, o-dichlorobenzene, dimethylformamide, dodecane, dimethyl sulfoxide, dioxane, ethylacetate, FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, methyl ethyl ketone, quinoline, cyclohexane

Chloroform**

m-cresol in chloroform, up to 10% hexafluoroisopropanol/chloroform

Dimethylformamide

dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene

o-Dichlorobenzene

1-chloronaphthalene, trichlorobenzene

TSKgel SuperH and Hhr²

Tetrahydrofuran³

acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride⁵, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine

Notes:

1) In case of TSKgel SuperHZ and Hxl, keep flow rate below <0.5 mL/min during solvent change. Solvent can be changed one way/one time only.
2) In case of TSKgel SuperH and Hhr, see Table 2 below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar solvent is not recommended.
3) All TSKgel Hxl, Hhr, SuperHZ, SuperH and GMH analytical columns are shipped containing tetrahydrofuran (THF), except TSKgel GMH-HT columns, which contain o-dichlorobenzene (ODCB).
4) THF in TSKgel G1000Hxl columns cannot be replaced with dichloromethane or dichloroethane.
5) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.

** TSKgel H columns may be specially ordered with this shipping solvent.

Table 2: Recommended flow rates (mL/min) for solvent exchange in TSKgel SuperH and Hhr columns
Solvent	SuperH (6.0mmIDx15cm)	Hhr (7.8mmIDx30cm)
n-Hexane	0.5	0.9
methyl ethyl ketone	0.4	0.7
dichloromethane, ethyl acetate	0.35	0.6
toluene, chloroform	0.3	0.5
dimethylformamide	0.2	0.4
carbon tetrachloride, pyridine	0.15	0.3
dimethyl sulfoxide, dioxane, ethanol, N-methylpyrrolidone, o-dichlorobenzene	0.1	0.2
quinoline, hexafluoroisopropanol, 1-chloronaphthalene	0.05	0.1