The TSKgel SuperH column line consists of eight columns with different pore sizes, TSKgel SuperH1000 through TSKgel SuperH7000, and four columns with an extended linear range of the calibration curve.  The calibration curves are shown below.

TSKgel SuperH-type columns are high efficiency/high throughput versions of the conventional Hhr columns. Both column types are based on the same bead chemistry.

Calibration curves on TSKgel SuperH columns

Main characteristics

• Maximum operating temperature for TSKgel SuperH-type columns is 140°C.
• Broad solvent range. All TSKgel SuperH-type columns are shipped in THF, which can be exchanged for a wide variety of organic solvents, as is shown below.
• TSKgel SuperH-type columns are high efficiency, high-throughput gel permeation (HT-GPC) columns with dimensions of 6 mm ID x 15 cm L.

Beware of extra-column bandbroadening

The volume of a TSKgel SuperH column is 3.4 times smaller than that of a conventional 7.8 mm L x 30 cm L column. As a result, peak volumes will be proportionally smaller on TSKgel SuperH-type columns compared to a corresponding TSKgel Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.

Columns with linear calibration curves

The TSKgel SuperH product line contains four linear or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHM-L, SuperHM-N, SuperHM-M, to SuperHM-H.

Solvent compatibility for TSKgel SuperH and Hhr columns The column shipping solvent limits the types of solvents that can be used with each column type.
Column type	Shipping solvent*	Can be replaced with
TSKgel SuperH and Hhr1	Tetrahydrofuran2	acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride5, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine

Recommended flow rates (mL/min) for solvent exchange in TSKgel Super H and Hhr columns
Solvent	TSKgel SuperH (6.0mmIDx15cm)	TSKgel Hhr (7.8mmIDx30cm)
n-Hexane	0.50	0.9
methyl ethyl ketone	0.40	0.7
dichloromethane, ethyl acetate	0.35	0.6
toluene, chloroform	0.30	0.5
dimethylformamide	0.20	0.4
carbon tetrachloride, pyridine	0.15	0.3
dimethyl sulfoxide, dioxane, ethanol, N-methylpyrrolidone, o-dichlorobenzene	0.10	0.2
quinoline, hexafluoroisopropanol, 1-chloronaphthalene	0.05	0.1

Recommended Solvents by Application for TSKgel H-type columns
Recommended solvent	Application
THF	polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5 mM LiBr	polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)	polyethylene, polypropylene
Chloroform	polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
m-Cresol/Chloroform	nylon, polyseter, polyamide, poly (ethylene terephthalate)
Toluene	polybutadiene, polysiloxane