Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers.

Maximum operating temperature for TSKgel Hxl-type columns is 60°C for TSKgel G1000 to G3000Hxl, and 80°C for the remaining columns in the product line. See TSKgel Hhr-type columns for operating temperatures of 140°C.

Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents (see Table below). 
   
Columns with linear calibration curves

Two of the linear columns are so-called mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The remaining column is a so-called multi-pore column, in which each particle contains a range of pore sizes that provide a linear calibration curve. The innovative multi-pore approach, pioneered by Tosoh, is a synthetic chemistry answer to the question of how to obtain a column with an extended linear calibration curve, while mixed-bed columns represent a mechanical way of obtaining a linear calibration curve. In general, Multipore columns have a smoother, more linear, calibration curve. See the product page for Multipore Hxl-M for the principles behind mixed-bed and multi-pore columns. 

Solvent compatibility for TSKgel Hxl (and TSKgel SuperHZ) columns The column shipping solvent limits the types of solvents that can be used with each column type.
Column type	Shipping solvent*	Can be replaced with
TSKgel Hxl1 and SuperHZ	Tetrahydrofuran2,3	benzene, chloroform, toluene, xylene, dichloromethane, dichloroethane
	Acetone**	carbon tetrachloride4, o-dichlorobenzene, dimethylformamide, dodecane, dimethyl sulfoxide, dioxane, ethylacetate, FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, methyl ethyl ketone, quinoline, cyclohexane
	Chloroform**	m-cresol in chloroform, up to 10% hexafluoroisopropanol in chloroform
	Dimethylformamide	dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene
	o-Dichlorobenzene	1-chloronaphthalene, trichlorobenzene

In case of TSKgel SuperHZ and Hxl, keep flow rate below <0.5 mL/min during solvent change. Solvent can be changed one way/one time only.

All TSKgel Hxl, Hhr, SuperHZ, SuperH and GMH analytical columns are shipped containing tetrahydrofuran (THF), except TSKgel GMH-HT columns, which contain o-dichlorobenzene.

THF in TSKgel G1000Hxl columns cannot be replaced with dichloromethane or dichloroethane.

Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.
** TSKgel H columns may be specially ordered with this shipping solvent.

Recommended solvents/Applications for TSKgel H-type columns
Recommended solvent	Application
THF	polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, poly(butadiene), polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5mM LiBr	polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)	polyethylene, polypropylene
Chloroform	polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
m-Cresol/Chloroform	nylon, polyester, polyamide, poly(ethylene terephthalate)
Toluene	polybutadiene, polysiloxane