TSKgel Super-ODS, Super-Octyl and Super-Phenyl columns are prepared from spherical 2 µm silica particles with 140 Å pore diameter. The silica beads in the columns are extremely uniform in size which provides column efficiencies of more than 160,000 plates per meter, yet have a pressure drop comparable to columns packed with 3 µm particles. The main advantages of the Super Series columns are savings in time and solvent usage, in addition to the improved quantitation resulting from reduced band broadening.
Caution: The silica particles in Super Series columns have a relatively small pore volume, which results in shorter retention times than obtained on most other reversed phase columns. For instance, to achieve similar retention times as obtained on TSKgel ODS-100V, the percentage organic solvent in the mobile phase has to be reduced by about 20-30% on TSKgel Super-ODS columns. | |
 Highlights |
-
The silica particles used in Super Series columns are uniform spherical 2 µm beads with 140 Å pores.
-
Pressure drop is not excessive due to the monodisperse particle size distribution.
-
Polymeric bonded phase structure and exhaustive endcapping effectively shields the solutes from interacting with residual silanol groups.
-
TSKgel Super Series require approximately 20% less organic solvent than other popular TSKgel reversed phase columns.
-
2 µm particles provide superior resolution and speed, as well as improved sensitivity. | |