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Tosoh Bioscience GmbH: The Chemistry of Innovation
Reversed Phase Chromatography
TSK-GEL Columns
HPLC Columns
Silica-based Columns
Polymer-based Columns
TSKgel Octadecyl-2PW
TSKgel Octadecyl-4PW
TSKgel Octadecyl-NPR
TSKgel Phenyl-5PW RP


TSKgel Phenyl-5PW RP offers similiar selectivity to it's hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients.

The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel PHenyl-5PW PR can withstand rigorous cleaning protocols using either acid or base. The 1000 Å pore size accommodates globular protein samples up to 1,000,000 Da.

 Structure:



 Product Attributes:
Pore size (mean): 1.000 Å

Exclusion Limit: 1,000,000 Da

Particle size (mean):  10, 13 and 20 μm

Functional group: Phenyl (monomeric bonding
chemistry)

 Literature:

Chromatography Catalog - Reversed Phase Chromatography

Reversed Phase Brochure

Operating Conditions and Specifications
TSKgel Phenyl-5PW RP


Application Note - HPLC analysis of lysozyme in different types of wine

 Application

Protein purification and purity check on
TSKgel Phenyl-5PW RP




Ordering information:
To place an order please contact
Customer Service

 
P/N Description Pore size Particle size 

Dimension

14007 TSKgel Phenyl-5PW RP, Glass 1.000 Å

10 μm 8 mm ID x 7.5 cm L


18756 TSKgel Phenyl-5PW RP 1.000 Å 10 μm 2 mm ID x 7.5 cm L


08043 TSKgel Phenyl-5PW RP 1.000 Å  10 μm 4.6 mm ID x 7.5 cm L


16260 TSKgel Phenyl-5PW RP 1.000 Å 13 μm 21.5 mm ID x 15 cm L


42159 TSKgel Phenyl-5PW RP Guard Cartridge for P/N
18756, 3 pieces/ pkg
1.000 Å   10 μm 2 mm ID x 1 cm L


19308 TSKgel Guard Cartridge Holder for P/N 42159     2 mm ID x 1 cm L


19007 TSKgel Guard Cartridges for P/N 08043,
3 pieces/ pkg, Replaces P/N 14126
1.000 Å  10 μm 3.2 mm ID x 1.5 cm L


19018 TSKgel Guard Cartridge Holder for P/N 19007,
Replaces P/N 14100
    3.2 mm ID x 1.5 cm L




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Technical support

Frequently asked questions
-- RPC


Search the Tosoh HPLC Application Database

General Principles of
Chromatography - Mode Poster


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Tosoh Bioscience GmbH Zettachring 6, 70567 Stuttgart, Germany  Tel: +49-711-132570  Email: info.tbg@tosoh.com