Polymeric Columns

Polymethacrylate-based reversed phase columns are available in a range of pore and particle sizes. Although often not as efficient and less robust than silica-based RPC columns, key advantages of polymer-based columns are:

Wider pH range (1 to 12) allows many basic compounds to be analyzed in their uncharged form, thus reducing secondary adsorption and improving peakshape.

Improved recovery for peptides and proteins due to reduced secondary interactions.

Highlights

Polymer-based RPC columns are chemically stable from pH 2-12, allowing operation at basic pH where silica-based columns have limited chemical stability.
Polymer-based TSKgel RPC columns can be cleaned and endotoxin removed by using either strong acid or base.
Polymer-based TSKgel RPC columns are available packed with nonporous resins (NPR) or with porous resins of various sizes. The proper column to use is selected based on sample MW or application.

TSKgel Octadecyl-NPR	TSKgel Octadecyl-2PW	TSKgel Octadecyl-4PW	TSKgel Phenyl-5PW RP
2.5 µm particle size fast kinetics, high column efficiency, quantitative protein recovery at sub-µg loads.	TSKgel Octadecyl-2PW is based on 5 µm particle size G2000PW resin, with 125 Å pores.	TSKgel Octadecyl-4PW is based on 7 µm particle size G4000PW resin, which contains 500 Å pores.	TSKgel Phenyl-5PW RP is based on 10 µm particle size G5000PW resin, which has an average pore size of 1000 Å.

General Principles of
Chromatography - Mode Poster

Reversed Phase Brochure

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