Reversed Phase HPLC Columns

Starting in the mid 1970s Reversed Phase Liquid Chromatography (RPC) has become the standard technique to analyze small molecular weight compounds in industrial, academic and governmental laboratories.

More recently, RPC has become the accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.

Tosoh Bioscience offers 15 distinct Reversed Phase column types which are either based on silica or methacrylate particles as discussed below.

Silica-based Columns	Methacrylate-based Columns
The silica-based TSKgel reversed phase column products consist of eleven stationary phases over 4 key product families. Each TSKgel silica column features:	The polymer-based TSKgel reversed phase columns are synthesized from polymethacrylate polymers in a range of pore and particle sizes. Advantages of polymethacrylate particles include:
High purity Type B Silica High efficiencies Excellent recoveries Low bleed for MS	Hydrophilic backbone to improve recovery and reduce secondary interactions Stable from pH 2.0 to 12.0 Compatibility with organic solvents eliminates swelling
New TSKgel ODS-100V and 100Z (100 Å) Monomeric Bonded Silica (80 Å) High Efficiency Specialty Silica Columns TSKgel ODS-140HTP for high throughput applications NEW TSKgel Protein C4-300 columns	TSKgel Octadecyl-2PW (125 Å) TSKgel Octadecyl-4PW (500 Å) TSKgel Phenyl-5PW RP (1000 Å) TSKgel Octadecyl-NPR (nonporous)

General Principles of
Chromatography - Mode Poster

Reversed Phase Brochure

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