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Tosoh Bioscience offers a broad line of high efficiency columns for analysis and isolation of biomolecules by ion exchange chromatography. For Anion as well as Cation Exchange Chromatography, the product line contains methacrylate-based and silica-based columns for the analysis of proteins, peptides, DNA- and RNA- derived oligonucleotides and other nucleic acid fragments.
Polystyrene-based ion exchange columns are available for analyzing low MW sugars, amino acids, nucleic acid bases, and small drug candidates.
Specialty columns are available for organic acids by ion-exclusion (SCX) and mono- and disaccharides (Sugar AXI and Sugar AXG).
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 Common Characteristics of TSK-GEL Anion and Cation Exchangers |
The methacrylate base resin, G5000PW (5PW), is a spherical 10 μm particle with approximately 1000 Å pores. TSK-GEL 5PW is derivatized with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl (CM) functionalities to provide a weak anion, a strong cation, and a weak cation exchanger, respectively. The polyamine chemistry employed in TSKgel SuperQ-5PW results in a high capacity strong anion exchanger with a smaller effective pore size than TSKgel DEAE-5PW.
Particles in TSKgel BioAssist Q columns contain very large pores (~4000 Å) that are functionalized with polyamine groups to form a network structure. The capacity of BioAssist Q columns is high over a wide molecular weight range. TSK-GEL BioAssist S columns are packed with particles possessing 1300 Å pores functionalized with sulfopropyl groups.
Methacrylate is also the backbone of nonporous resin (NPR) and TSK-GEL STAT columns. Nonporous CM-STAT, SP-STAT, Q-STAT and DNA-STAT columns combine high densites of functional groups with relatively large particle sizes. Due to their moderate backpressure and good sample capacities they are ideally suited for fast separations. DEAE-NPR, DNA-NPR and SP-NPR are packed with 2.5 μm particles. High column efficiency coupled with low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation. Due to the absence of large pores, protein recovery is generally very high on NPR columns.
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| Anion Exchange Columns (type, matrix) |
Cation Exchange Columns (type, matrix) |
Strong anion, methacrylic
TSKgel BioAssist Q
TSKgel SuperQ-5PW
TSKgel Q-STAT
TSKgel DNA-STAT
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Strong cation, methacrylic
TSKgel BioAssist S
TSKgel SP-5PW
TSKgel SP-NPR
TSKgel SP-STAT
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Strong cation, silica
TSKgel SP-2SW
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Strong anion, polystyrene
TSKgel Sugar AXI
TSKgel Sugar AXG
TSKgel SAX
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Strong cation, polystyrene
TSKgel SCX |
Weak anion, methacrylic
TSKgel DEAE-5PW
TSKgel DEAE-NPR
TSKgel DNA-NPR
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Weak cation, methacrylic
TSKgel CM-5PW
TSKgel CM-STAT |
Weak anion, silica
TSKgel DEAE-2SW
TSKgel DEAE-3SW
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Weak cation, silica
TSKgel CM-2SW
TSKgel CM-3SW
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