Tosoh Bioscience offers a broad line of high efficiency columns for analysis and isolation of biomolecules by ion exchange chromatography. For Anion as well as Cation Exchange Chromatography, the product line contains methacrylate-based and silica-based columns for the analysis of proteins, peptides, DNA- and RNA- derived oligonucleotides and other nucleic acid fragments.
Polystyrene-based ion exchange columns are available for analyzing low MW sugars, amino acids, nucleic acid bases, and small drug candidates.
Specialty columns are available for organic acids by ion-exclusion (TSKgel SCX) and mono- and disaccharides (TSKgel Sugar AXI and Sugar AXG).
| Common Characteristics of TSKgel Anion and Cation Exchangers|
The methacrylate base resin, G5000PW (5PW), is a spherical 10 μm particle with approximately 1000 Å pores. TSKgel 5PW is derivatized with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl (CM) functionalities to provide a weak anion, a strong cation, and a weak cation exchanger, respectively. The polyamine chemistry employed in TSKgel SuperQ-5PW results in a high capacity strong anion exchanger with a smaller effective pore size than TSKgel DEAE-5PW.
Particles in TSKgel BioAssist Q columns contain very large pores (~4000 Å) that are functionalized with polyamine groups to form a network structure. The capacity of TSKgel BioAssist Q columns is high over a wide molecular weight range. TSKgel BioAssist S columns are packed with particles possessing 1300 Å pores functionalized with sulfopropyl groups.
Methacrylate is also the backbone of nonporous resin (NPR) and TSKgel STAT columns. Nonporous TSKgel CM-STAT, SP-STAT, Q-STAT and DNA-STAT columns combine high densites of functional groups with relatively large particle sizes. Due to their moderate backpressure and good sample capacities they are ideally suited for fast separations. TSKgel DEAE-NPR, DNA-NPR and SP-NPR are packed with 2.5 μm particles. High column efficiency coupled with low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation. Due to the absence of large pores, protein recovery is generally very high on NPR columns.
| Anion Exchange Columns (type, matrix)
|| Cation Exchange Columns (type, matrix)|
|Strong anion, methacrylic
TSKgel BioAssist Q
|Strong cation, methacrylic|
TSKgel BioAssist S
||Strong cation, silica|
|Strong anion, polystyrene
TSKgel Sugar AXI
TSKgel Sugar AXG
|Strong cation, polystyrene|
|Weak anion, methacrylic
|Weak cation, methacrylic|
|Weak anion, silica
|Weak cation, silica|
General Principles of
Chromatography - Mode Poster
Characterizing Biotherapeutics with HPLC - smart solutions for large molecules - Application note
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